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Composite

Part:BBa_K560000:Design

Designed by: Zhang Chao   Group: iGEM11_WHU-China   (2011-09-25)


Blue Light Sensor+PtrpL+mRFP1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 595


Design Notes

This system had better work in TrpR mutant E.coli strain.


Source

LovTAP was assembled by Strickland et al.

References